Abstract: This paper studied the relation between porcine serum protein and ammonium sulfate saturation when salt fractionation by Polyacryamide Gel Electrophoresis ,the result showed the main deposition scope of porcine serum protein is 25%～70% ammonium sulfate saturation ,each porcine serum protein has respective deposition scope. The best ammonium sulfate fractionation method of decuple dilute porcine serum albumin is pH7.4, adjust ammonium sulfate saturation to 50%, remove deposition by centrifugation , and adjust the pH of clear liquid to 4.5, the ammonium sulfate saturation to 55% ,then albumin was deposited. In the porcine serum protein which was deposited by 35% saturation of ammonium sulfate when pH is 7.4, the IgG yield rate is 69.5% and purity is 76%, when the ammonium sulfate saturation is 40%, the IgG yield rate is 91.8% and purity is 61.6%.

Key words: Polyacryamide Gel Electrophoresis, porcine serum, ammonium sulfate, albumin, immunoglobulin G(IgG)