FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (3): 181-184.

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Separation, Purification and Composition Analysis of Polysaccharides in Leaves of Morus alba L.

 OU Yang-Zhen , CHEN  Jun, LI  Yong-Hui   

  1. School of Biological and Environmental Engineering, Jiangsu University
  • Online:2005-03-15 Published:2011-09-19

Abstract: The crude polysaccharides from Leaves of Morus alba L. were obtained by extracting hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides and then the crude Morus polysaccharides (MP1) were Isolated and purified by DEAE-cellulose column and Sephadex G-100 gel column chromatography. The result showed that MP1 was composed of three pure polysaccharides (MP11,MP12,Mp13). After converting the hydrolyzed polysaccha- ride into acetylated aldononitriles it was analysis by gas chromatography. The chemical components and the ratio showed follows:MP11:Rha:Ara:Xyl:Man:Glu:Gal=21:16:3:3:1:20;MP12:Rha:Glu=3:1;MP13’s component was mainly Rha. All these work could establish foundation for the further study of Morus Polysaccharides.

Key words: leaves of Morus alba L., polysaccharides, isolation purification, gaschromatography, component