Determining Isoflavones by the Emendation Method in Ultraviolet Spectrophotometry

Abstract

Abstract: Based on genistein as reference standard, the isoflavones content of standard mixture of genistein and daidzein was determined by ultraviolet spectrophotometry. The results showed that determination precision (determined value /standard value) was affected by the ratio of genistein and daidzein in the mixture, and the linear correlation existed between determination precision and A262/A250 absorbance, which could be used as a method to emendate the determined value. The average recovery rate by the method of pre-emendation and emendation was 91.8% and 97.3% respectively.

Key words: soybean, isoflavones, ultraviolet spectrophotometry, determination

SUN Ling, WEI Zhen-Cheng, ZHANG Ming-Wei. Determining Isoflavones by the Emendation Method in Ultraviolet Spectrophotometry[J]. FOOD SCIENCE, 2005, 26(3): 209-211.

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Determining Isoflavones by the Emendation Method in Ultraviolet Spectrophotometry

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