RP-HPLC Studies on Quantitative Determination of Carnosic Acid in Rosemarinus officinalis L

Abstract

Abstract: To establish a HPLC method for the separation and determination of Carnosic acid in Rosemarinus officimalis L, the Pheromenex C18 reverse Phase column (250mm×4.6mm, 5μm) and acetonitrile-methanol as mobile phase were used with flow rate 1.0ml/min detecting spectrum 210 nm and quantitating value by external standard method. The standard curve of linear relationship was of 20～200μg/ml and r=0.9998.The average recovery was 98.6%. The established method was accurate, sensitive, simple and reliable for the determination of Carnosic acid in Rosemarinus officinalis L.

Key words: RP-HPLC, Rosemarinus officinalis L, Carnosic acid

LIANG Zhen-Yi, HUANG Guang-Min, ZHANG De-La, LIU Chang-Sheng, CHEN Lei, CHEN Zong-Xian. RP-HPLC Studies on Quantitative Determination of Carnosic Acid in Rosemarinus officinalis L[J]. FOOD SCIENCE, 2005, 26(4): 203-205.

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RP-HPLC Studies on Quantitative Determination of Carnosic Acid in Rosemarinus officinalis L

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