Study on Isolation and Purification of Procyanidins from Seabuckthorn Fruit

Abstract

Abstract: The procyanidins from seabuckthorn fruit was isolated and purified with macroporous resin, Sephadex LH-20 and RP-HPLC. The result showed that procyanidins yield in elution aqueous was 71.9% while macroporous resin was eluted with 50% ethanol aqueous. The elution aqueous was further purified by Sephadex LH-20 with 50% ethanol/water(V/V) as mobile phase, procyanidins yield in elution aqueous was above 95%. The purified product was further analysed by RP-HPLC. It indicated that dimeric procyanidins were existed.

Key words: seabuckthorn fruit, procyanidins, isolated and purified, macroporous resin, Sephadex LH-20

ZHANG Chi, XU Xiao-Yun, PAN Si-Yi. Study on Isolation and Purification of Procyanidins from Seabuckthorn Fruit[J]. FOOD SCIENCE, 2005, 26(5): 183-185.

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Study on Isolation and Purification of Procyanidins from Seabuckthorn Fruit

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