Abstract: After being extracted by acetonitrile, cleaned by n-hexane liquid-liquid extraction and solid phase extration(SPE), fifteen sulphonamides residues in animal tissues could be separated on Agilent C18 column using acetonitrile - 0.02% H3PO4 with gradient elution as the mobile phase at a flow rate of 1.0 ml/min, and then simultaneously detected at 268 nm, the column temperature was at 30 ℃. The recovery rates of sample were in the range of 80.9%～92.3%, fifteen sulphonamides residues could be separated completely ,which proved to be accurate and reliable.

Key words: RP-HPLC, animal tissues, solid phase extraction(SPE), sulphonamides residue