Abstract: Objective: Study on the chemical constituents and contain cents of essential oil and fatty acids from the seeds ofdifferent species of Raphanus Sativus L. with GC and GC-MS. Methods: The essential oils were obtained by the agent extractionand the chemical compositions were separated by GC. The relative contents in the essential oil were determined by areanomalization and NIST and WILEY library searched and 14 were separated and 8 were identified. Result:The research resultexpresses, the seeds of different species of Raphanus Sativus L. contain the oil high( ≥30% ) and contain more the unsaturated fatty acids in the fats, have the bigger development application value.

Key words: seeds of different species of Raphanus Sativus L., fat, fatty acids, chemical constituents, GC-MS