FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (8): 318-320.

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A Simplified Method for Extracting DNA from the Mycelium of Edible Fungi

 TONG  Li-Hua, LIAN  Bin   

  1. Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University
  • Online:2005-08-15 Published:2011-09-19

Abstract: By means of tissue isolation, pure cultured mycelium was obtained from fruit body of Suillus bovines, which is akindo f ectomycorrhizal fungia ndc annot bec ultureda rtificially.T het raditional morphologicalm ethodsf ort hei dentificationo fS. bovinus are problematical, so we used analysis of rDNA internal transcribed spacer (ITS) sequences to identify S. bovinus andestablished the evolution tree for preliminary studying phylogenetic relationships of S.b ovinus.

Key words: Suillus bovinus, molecular identification, phylogenetic tree