Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

doi:10.7506/spkx1002-6630-201009041

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 182-186.doi: 10.7506/spkx1002-6630-201009041

• Bioengineering • Previous Articles Next Articles

Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

XIE Li-yuan1,2，ZHANG Yong3,*，DENG Ke-jun3，PENG Wei-hong1，GAN Bing-cheng1，LI Hong-jun2

1. Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China；2. College of Food
Science, Southwest University, Chongqing 400716, China；3. School of Life Science and Technology, University of Electronic
Science and Technology of China, Chengdu 610054, China

Received:2009-08-17 Online:2010-05-01 Published:2010-12-29
Contact: ZHANG Yong E-mail:zhangyong916@126.com

Abstract

Abstract:

In this study, the ribosomal DNA internal transcribed spacer (rDNA ITS) region of six species of Sanghuang was cloned and sequenced, and the characteristics of ITS sequences were compared and analyzed. The ITS sequences exhibited homology through BLAST analysis of GenBank database and a phylogentic tree was constructed. Results demonstrated that phylogenetic relationship of Sh-03, 04, 05 and 06 was close to Phellinus linteus, and phylogenetic relationship of Sh-01 was close to Phellinus baumii; but Sh-02 had distant phylogenetic relationship with Phellinus baumii, Phellinus linteus and Phellinus igniarius. Therefore, the characteristics of rDNA ITS sequences can be used as an accurate, reliable and straightforward method to identify the original species of Sanghuang and explore genetic diversity.

Key words: Sanghuang, Phellinus baumii, Phellinus linteus, Phellinus igniarius, ribosomal DNA internal transcribed spacer, molecular identification

CLC Number:

TS201.3

XIE Li-yuan1,2，ZHANG Yong3,*，DENG Ke-jun3，PENG Wei-hong1，GAN Bing-cheng1，LI Hong-jun2. Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis[J]. FOOD SCIENCE, 2010, 31(9): 182-186.

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Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

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