Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

doi:10.7506/spkx1002-6630-201009041

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (9): 182-186.doi: 10.7506/spkx1002-6630-201009041

• Bioengineering • Previous Articles Next Articles

Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

XIE Li-yuan1,2，ZHANG Yong3,*，DENG Ke-jun3，PENG Wei-hong1，GAN Bing-cheng1，LI Hong-jun2

1. Soil and Fertilizer Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China；2. College of Food
Science, Southwest University, Chongqing 400716, China；3. School of Life Science and Technology, University of Electronic
Science and Technology of China, Chengdu 610054, China

Received:2009-08-17 Online:2010-05-01 Published:2010-12-29
Contact: ZHANG Yong E-mail:zhangyong916@126.com

Abstract

Abstract:

In this study, the ribosomal DNA internal transcribed spacer (rDNA ITS) region of six species of Sanghuang was cloned and sequenced, and the characteristics of ITS sequences were compared and analyzed. The ITS sequences exhibited homology through BLAST analysis of GenBank database and a phylogentic tree was constructed. Results demonstrated that phylogenetic relationship of Sh-03, 04, 05 and 06 was close to Phellinus linteus, and phylogenetic relationship of Sh-01 was close to Phellinus baumii; but Sh-02 had distant phylogenetic relationship with Phellinus baumii, Phellinus linteus and Phellinus igniarius. Therefore, the characteristics of rDNA ITS sequences can be used as an accurate, reliable and straightforward method to identify the original species of Sanghuang and explore genetic diversity.

Key words: Sanghuang, Phellinus baumii, Phellinus linteus, Phellinus igniarius, ribosomal DNA internal transcribed spacer, molecular identification

CLC Number:

TS201.3

XIE Li-yuan1,2，ZHANG Yong3,*，DENG Ke-jun3，PENG Wei-hong1，GAN Bing-cheng1，LI Hong-jun2. Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis[J]. FOOD SCIENCE, 2010, 31(9): 182-186 https://doi.org/10.7506/spkx1002-6630-201009041

[1]	SHI Wanrong, SHEN Shuyue, WANG Qin, LI Zhengpeng, LI Tingting. Origin Traceability and Cultivation Mode Identification of Phellinus linteus Based on Hyperspectral Imaging Combined with Deep Learning [J]. FOOD SCIENCE, 2026, 47(6): 342-350.
[2]	CHEN Wenqiang, WANG Xiaofu, CHEN Xiaoyun, PENG Cheng, XU Junfeng, CAI Jian. Evaluation of the Ability of Different Plant DNA Barcodes to Identify Dendrobium officinale [J]. FOOD SCIENCE, 2021, 42(22): 131-139.
[3]	HU Xiaotong, YE Yujie, SHI Guang, ZHAO Nanxi, GU Mingliu, YAN Yuning, ZHOU Jianing, AN Liping. Extraction of Polysaccharides from Fruiting Bodies of Phellinus igniarius and Its Protective Effect on D-galactose Induced 3T3 Cell Injury [J]. FOOD SCIENCE, 2020, 41(19): 204-211.
[4]	YE Yujie, SHI Guang, ZHOU Zhengyi, WANG Jiaqi, AN Liping, DU Peige. Comparative Chemical Composition of Mycelium and Fruit Body of Phellinus igniarius [J]. FOOD SCIENCE, 2019, 40(24): 246-251.
[5]	QIAN Hua, CHEN Bin, HUANG Xiaode, ZHU Yuyao, ZHAO Botao. Effect of Different Cell Wall Disruption Techniques on the Extraction Yields of Functional Components from Fruit Bodies of Phellinus linteus [J]. FOOD SCIENCE, 2016, 37(10): 23-27.
[6]	ZHOU Huiji, MA Haile, GUO Danzhao, WANG Zhenbin. Physicochemical Properties and Antioxidant Activity of Intracellular Polysaccharides from Phellinus igniarius Precipitated by Different Ethanol Concentrations [J]. FOOD SCIENCE, 2015, 36(19): 34-38.
[7]	ZHAN Dong-rui，MENG Xiao-lu，LI Lian-qiang，CAO Na，WANG Hui，LIU Tian-xing，XIN Zhi-hong*. Isolation and Identification of Endophytic Fungal Strain Salicorn 35 from Salicornia bigelovii and Optimization of Its Fermentation Conditions for Production of Antioxidants [J]. FOOD SCIENCE, 2013, 34(19): 158-165.
[8]	YANJing-kun，MAHai-le，ZHUZi-ping，ZHENGHui-hua，YANGXiao-ming，LIUWei-min. Physico-chemical Properties and Antioxidant Activity in vitro of Intracellular Polysaccharides from Phellinus igniarius [J]. FOOD SCIENCE, 2012, 33(9): 36-40.
[9]	GUODong-qin，DAIMu-lan，ZHOUZhi-qin，JIAOBi-ning，YANGXiao-hong. Molecular Identification and Morphological Analysis of Yeasts in Spoiled Orange Juice [J]. FOOD SCIENCE, 2012, 33(9): 131-137.
[10]	SHAO Jie, LUO Jian-guang, ZENG Xiao-xiong. Optimization of Cultivation Conditions for Extracellular Polysaccharide Production by P. baumii Pilát [J]. FOOD SCIENCE, 2012, 33(3): 121-125.
[11]	XIE Li-yuan，ZHANG Yong，PENG Wei-hong，GAN Bing-cheng. Immune Function and Antioxidant Activity of Intracellular Polysaccharides from Phellinus baumii [J]. FOOD SCIENCE, 2011, 32(9): 276-281.
[12]	HU Qin，YANG Xiao-hong，GUO Dong-qin，LIU Chao-yi，ZHOU Zhi-qin，JIAO Bi-ning. Morphological and Molecular Identification of Two Opportunistic Pathogenic Yeast Strains in Orange Juice [J]. FOOD SCIENCE, 2011, 32(9): 231-235.
[13]	XIE Li-yuan,TAN Wei,GUO Yong,JIA Ding-hong,PENG Wei-hong,GAN Bing-cheng. Application of Response Surface Methodology for Optimization of Liquid Fermentation Medium for Intracellular Polysaccharide Production by Phellinus baumii [J]. FOOD SCIENCE, 2011, 32(7 ): 224-228.
[14]	GAO Xing-xi1,YAO Qiang2,GONG Zhi-yuan2,LANG Xiao1,REN Peng-fei2,LIU Yan3. Effect of Different Fatty Acids on Liquid-state Fermentation of Phellinus igniarius [J]. FOOD SCIENCE, 2011, 32(7 ): 198-201.
[15]	XIE Li-yuan1， ZHANG Yong2,*，GUO Yong1，WU Xiang1，PENG Wei-hong1，GAN Bing-cheng1. Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii [J]. FOOD SCIENCE, 2011, 32(5 ): 143-147.

Molecular Identification of Medicinal Fungus Sanghuang based on rDNA ITS Sequence Analysis

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments