Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii

doi:10.7506/spkx1002-6630-201105032

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 143-147.doi: 10.7506/spkx1002-6630-201105032

• Basic Research • Previous Articles Next Articles

Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii

XIE Li-yuan1， ZHANG Yong2,*，GUO Yong1，WU Xiang1，PENG Wei-hong1，GAN Bing-cheng1

1. Institute of Soil and Fertilizer, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China；
2. School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China

Received:2010-06-19 Revised:2011-02-14 Online:2011-03-15 Published:2011-03-03
Contact: XIE Li-yuan1 E-mail:xieliyuan77@163.com

Abstract

Abstract: The isolation, purification and physi-cochemical characterization of mycelial polysaccharides from Phellinus baumii were studied. Two polysaccharide fractions were separated from crude deproteinized polysaccharides from Phellinus baumii mycelia by DEAE-52 cellulose column chromatography, followed by purification on Sephadex G-100 gel filtration column. The two fractions were determined by HPLC to contain neither protein nor nuclear acid and to be pure non-starch polysaccharides, their molecular weights were 4.74×104 D and 8.71×103 D, respectively, and they were both composed of rhamnose, arabinose, xylose, galactose and glucose in a molar ratio of 3.47:1.99:1:63.27:13.44 and 10.46:1:1.03:182.75:30.94, respectively.

Key words: Phellinus baumii, polysaccharide, purification, physico-chemical characteristics

CLC Number:

O636.1

XIE Li-yuan1， ZHANG Yong2,*，GUO Yong1，WU Xiang1，PENG Wei-hong1，GAN Bing-cheng1. Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii[J]. FOOD SCIENCE, 2011, 32(5 ): 143-147.

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Isolation, Purification and Physico-chemical Characteristics Analysis of Mycelial Polysaccharides from Phellinus baumii

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