Abstract: The extraction of resveratrol from waste residue of grape spike-stalk was investigated. The yield of resveratrol was determined by ultraviolet spectrometry. The effect of different solvents such as methanol, ethanol, acetone and acetic ether on extraction yield was studied. Besides, the effects of volume fraction of ethanol, radio of solid/liquid,extraction temperature, extraction time, and extraxtion times on extraction yield of resveratrol were studied . On the basis of single factor and orthogonal test, the optimum condition were determined as follows: volume fraction of 50% ethanol solution as extractive solvent, extraction temperature 70 ℃, radio of solid/liquid 1:15, extraction time 4.0h, the mesh of material 60～100 mesh, the extraction yield of resveratrol could reach 0.34% one time.

Key words: resveratrol, grape spike-stalk, extraction, ultraviolet spectrometry