HPLC Determination of Effective Components in Soybean Isoflavones Microencapsule

Abstract

Abstract: Objective: To establish a method for the determination of effective component in soybean isoflavones microencapsule. Methods: The HPLC system consisted of Inertsil ODS-3 column(150 mm×4.6 mm, 5μm), column temperature at 40℃, with 1.5% acetic acid - methanol-acetonitrile (62:18:20) as mobile phase. Detection wavelength was at 260 nm . Samples were extracted by 70% ethanol with ultrasonic wave, and then filtrated with 0.45μm filtration membrane. Results: The separate rates of three component are all higher than 1.5. One determination was finished within 13 minutes. Three batches of sample were analyzed and compared with the raw material. The assays of daidzein, glycitein and genistein showed that there are ratio relationship before and after microencapsulated. Conclusion: The method is simple, rapid and applicable for the determination of soybean isoflavones microencapsule, and it provides a preferable assay method for the quality control of these health-care foodstuffs containing soybean isoflavones aglycones.

Key words: soybean isoflavones, microencapsule, wastewater of soybean production, HPLC, determination

XU Shi-Fang, CHEN Ai-Ying, JIANG Li-Xia. HPLC Determination of Effective Components in Soybean Isoflavones Microencapsule[J]. FOOD SCIENCE, 2007, 28(11): 473-475.

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HPLC Determination of Effective Components in Soybean Isoflavones Microencapsule

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