Study on Active Site of L-arabinose Isomerase from Lactobacillus plantarum

Abstract

Abstract: The modification chemicals of NBS, PMSF, DEPC, EDC, TNBS, 2-mercaptoethanol and PCMB were used to reactwith L-arabinose isomerase from Lactobacillus plantarum. The L-arabinose isomerase could be inactivated with NBS, PMSF and DEPC. The tryptophan residues, serine residues and histidine residues might be involved in the active site of the enzyme. 4 mg/ml galactose could completely inhibit the inactivation of L-arabinose isomerase with NBS and PMSF, but the substrate showed no effect on the modification by DEPC. The results revealed that serine and tryptophan are positioned in the substrate binding site while histidine in the catalytic site.

Key words: Lactobacillus plantarum, L-arabinose isomerase, chemical modification, active site

ZHANG Hua, JIANG Bo, PAN Bei-Lei. Study on Active Site of L-arabinose Isomerase from Lactobacillus plantarum[J]. FOOD SCIENCE, 2007, 28(12): 340-344.

[1]	WANG Chunchun, QIN Xiaoli, KAN Jianquan, LIU Xiong, SUO Huayi, ZHONG Jinfeng. Comparison of Reactive Sites of Different Fatty Acid Molecules by Quantum Chemistry Calculation [J]. FOOD SCIENCE, 2021, 42(8): 74-80.
[2]	LI Xinrui, ZHAO An, FAN Xiaopiao, GAO Wenwen, SHANG Jiacui, ZHAO Penghao, ZHAO Le, ZHOU Xue, MENG Xiangchen. Prokaryotic Expression and Purification of PurR and PurL from Lactobacillus plantarum KLDS1.0391 and Their Interaction with Bacteriocin Synthesis Promoter [J]. FOOD SCIENCE, 2021, 42(6): 75-81.
[3]	WU Lingfeng, WEI Shuang, CHEN Ying, DU Minjie, LIU Sixin, WANG Lu, LI Congfa. Effects of Fructooligosaccharides on the Chemical, Microbiological and Antioxidant Properties of Tamarind (Tamarindus indica L.) Juice Fermented by Lactobacillus plantarum A33 [J]. FOOD SCIENCE, 2021, 42(4): 122-129.
[4]	LEI Wenping, ZHOU Hui, CHEN Qi, ZHOU Xingrong, WU Kun, WANG Jiaqi, LIU Chengguo. Screening and Identification of Probiotics with Hyaluronidase Inhibitory Activity in Vitro [J]. FOOD SCIENCE, 2021, 42(2): 151-157.
[5]	ZHAO Le, ZHANG Xiaotong, LIU Lijun, XIE Shuiqi, JIN Qiwen, MENG Xiangchen. Effects of Amino Acids on Growth and Bacteriocin Synthesis of Lactobacillus plantarum KLDS1.0391 [J]. FOOD SCIENCE, 2021, 42(18): 37-44.
[6]	XIAO Ye, YE Jingqin, LI Xiaochen, LI Xiaohui, SHI Wenzheng, LU Ying. Effect of Lactobacillus plantarum on Reducing the Immunological Activity of Tropomyosin [J]. FOOD SCIENCE, 2021, 42(18): 95-102.
[7]	LIU Jingbo, WANG Ziqin, YU Yiding, ZHANG Ting, LIU Boqun. Identification and Mechanism Analysis of Angiotensin-I Converting Enzyme Inhibitory Peptides from Soybean Meal [J]. FOOD SCIENCE, 2021, 42(12): 123-129.
[8]	GAO Zhaojian, HUANG Lianghao, DING Feihong, ZHAO Yifeng, CHEN Teng. Characterization of Novel Broad-Spectrum Bacteriocin Produced by Lactobacillus plantarum from Pickles [J]. FOOD SCIENCE, 2021, 42(10): 171-177.
[9]	FANG Laishan, LAI Qiang, ZHONG Zemin, HUANG Yumao. Sequence Analysis of an Endogenous Plasmid in Lactobacillus plantarum and Construction of a Shuttle Expression Vector Using It [J]. FOOD SCIENCE, 2020, 41(4): 118-124.
[10]	LU Zhou, DAI Yiqiang, Hafiz Abdul RASHEED, WU Han, XIA Xiudong, DONG Mingsheng. Antimicrobial Activity of Soy Whey Fermented by Lactobacillus plantarum D1501 and Purification and Identification of Bacteriocin from It [J]. FOOD SCIENCE, 2020, 41(24): 117-124.
[11]	ZHANG Yuzhu, WANG Bingyi, ZENG Zi’ao, XU Ruoyun, LI Jun, FAN Mingtao, WEI Xinyuan. Effect of Culture Conditions on the Formation of Selenium Nanoparticles with Different Particle Sizes by Microbial Reduction Using Lactobacillus plantarum and Their Bioactivity Evaluation [J]. FOOD SCIENCE, 2020, 41(22): 119-126.
[12]	LI Qiangkun, LIU Chenjian, LUO Yiyong, YANG En, LI Xiaoran. Effects of Different Fermentation Conditions on Folate Synthesis of Lactobacillus plantarum [J]. FOOD SCIENCE, 2020, 41(2): 114-118.
[13]	GUO Lidong, ZHANG Wenwen, LIU Yan, HUANG Mengling, MEN Yue, ZHANG Yanli, WANG Liqun. Isolation and Probiotic Properties of Lactobacillus plantarum HUCM115 from Traditional Pickled Chinese Cabbage [J]. FOOD SCIENCE, 2020, 41(18): 140-145.
[14]	LIU Yaoyao, LI Lu, LIU Tingting, ZHANG Shanshan, XUN Yiping, ZHU Hong, WANG Shijie, BIAN Yanqing, ZHAO Baohua. Effect of Lactobacillus plantarum Powder on Intestinal Mucosal Barrier Function in Obese Rats [J]. FOOD SCIENCE, 2020, 41(17): 153-160.
[15]	WANG Hongmei, JIANG Sirui, TAO Yang, HAN Yongbin. Effect of Ultrasound on Microbial Growth and Metabolisms of Various Components including Phenolics during Fermentation of Apple and Strawberry Juice by Lactobacillus plantarum [J]. FOOD SCIENCE, 2020, 41(14): 72-81.

Study on Active Site of L-arabinose Isomerase from Lactobacillus plantarum

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments