FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (12): 74-77.
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LI Nan, XIONG Bin, ZHANG Yun-Kai, ZHENG Xiao-Xian, LIANG Zhi-Qun
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Abstract: The yeast strain with high activity of producing phenylalanine ammonia-lyase, SA1, was screened from soil. The optimum enzyme productivity and conditions for the conversion of trans-cinnamic acid into L- phenylalanine were determined. The results showed that the production of enzyme was kept high by addition of L-phenylalanine, L-tyrosine and isoleucine, where L- tyrosine was optimum. The optimal parameters of the conversion reaction were: 2.0% trans-cinnamic acid, NH4HCO3:NH•3H2O (W:V)=4:4, pH10.6, 34℃, and the time of reaction 8h. The enzyme activity was kept stable by the addition of glycerol.
Key words: L-phenylalanine, phenylalanine ammonia-lyase, trans-cinnamic acid
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