FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (7 ): 255-257.doi: 10.7506/spkx1002-6630-201107055

• Bioengineering • Previous Articles     Next Articles

Cloning and Sequence Analysis of Phenylalanine Ammonia-lyase (PAL) Gene from Fagopyrum esculentum

LI Cheng-lei,MENG Hua,ZHANG Xiao-wei,CHEN Hui,SHAO Ji-rong,WU Qi*   

  1. College of Life Science, Sichuan Agricultural University, Ya’an 625014, China
  • Received:2010-07-05 Revised:2011-02-24 Online:2011-04-15 Published:2011-03-30

Abstract: The cDNA ORF sequence of phenylalanine ammonia-lyase gene (FePAL) from Fagopyrum esculentum was cloned by RT-PCR. The cDNA sequence was 2169 bp in length, encoding 722 amino acids and had a high homology (80%-97%) with PAL genes from other plants. The deduced amino acid sequence of FePAL contained the typical sequence (GTTTASGDLVPLSYIA) in PAL activity center region, deamination residues and catalytic active sites. Phylogenetic tree analysis revealed that FePAL was most closely related to the PAL of Fagopyrum tataricum.

Key words: Fagopyrum esculentum, phenylalanine ammonia-lyase gene, cloning, sequence analysis

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