FOOD SCIENCE ›› 2004, Vol. 25 ›› Issue (4): 25-28.

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Isolation and Characterization of Defatted Hua-Za 3 Meal Protein

 XUE  Zhao-Hui, WU  Mou-Cheng, YIN  Jing-Zhang, RUAN  Zheng   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University;2.Agronomy College, Xinjiang Agricultural University
  • Online:2004-04-15 Published:2011-10-24

Abstract: Classical fractionation protein of the undenatured Hua-Za 3 meal, which has been an increasingly important oilseedcrop in China, resulted in H2O-, 5% NaCl-, 70%EtOH-, and 0.4%NaOH-soluble fractions in ratios of 37:32:3:29 respectively.The major proteins in Hua-Za 3 meal were albumin and globulins. The molecular weight of fractionation was shown by SDS-PAGE. Amino acid profile of isolates indicated high nutrition quality for use in consumers’ products.

Key words: rapeseed protein isolate, amino acid, SDS-PAGE