FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (19 ): 240-243.doi: 10.7506/spkx1002-6630-200919055

Previous Articles     Next Articles

Effects of Turmeric rhizome Naphtha on Gene Expression of Hepatocarcinoma Cell SMMC-7721

WANG Shun-qi1,2,3，CHEN Li2，NI Hong2，MU Ya-li4，LIU Wei3，XIE Ming-yong1,*     

  1. 1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ； 2. College of Life
    Science, Nankai University, Tianjin 300071, China；3. School of Life Sciences and Food Engineering, Nanchang University, Nanchang
    330031, China；4. Institute of Computer Technology and Engineering, Nanchang University, Nanchang 330031, China
  • Received:2009-03-16 Online:2009-10-01 Published:2010-12-29
  • Contact: XIE Ming-yong

RichHTML

0

PDF (PC)

212

Abstract:

Objective: To detect effects of Turmeric rhizome naphtha (TRN) on cell growth and gene expression of hepatocarcinoma cell SMMC-7721. Methods: Cell growth of hepatocarcinoma cell SMMC-7721 with TRN treatment was evaluated by MTT assays, and gene expression of hepatocarcinoma cell SMMC-7721 with TRN treatment for 36 hours was explored with microarray of hepatocarcinoma-associated genes, which resulted from suppression subtractive hybridization library. Results: After TRN treatment on hepatocarcinoma cell SMMC-7721 for 36 hours, cell growth was greatly inhibited and gene expression profiling analysis indicated that 23 genes were up-regulated and 3 genes were down-regulated. Conclusion: The effect of TRN treatment on hepatocarcinoma cell SMMC-7721 can be achieved through alteration of multiple genes expression, which provides important clues to understand the molecular mechanism of TRN effects on hepatocarcinoma cells.

Key words: Turmeric rhizome naphtha (TRN), hepatocarcinoma cell SMMC-7721, MTT assays, microarray, gene expression

CLC Number: