Abstract: Chitosan was used to replace the substrate M.Lysodeikticus to determine the enzyme activity of lysozyme.It showedthat the optimum temperature and optimum pH were 55℃ and 4.5 respectively and it was accordant with the lamber-bier lawwhen the enzyme concentration was 0～4.5U/ml.The regression equation is Y=0.2173X-0.0114 with linear correlationcoefficient as 0.9989.

Key words: chitosan, lysozyme, enzyme activity, M.Lysodeikticus