Study on Interaction between Four Flavones and DNA

Abstract

Abstract: The interaction of morin,kaempferol,quercetin hydrate and myricetin with herring sperm DNA were studied in this paper by ultraviolet absorption spectrum,melting curve,and bingding constant,respectively. The investigation on the interaction of the four flavones and DNA indicates that the absorbance value at the maximum peak of DNA are increased,and the wavelength number at the maximum peak are blue-shifted for morin and kaempferol,and red-shifted for quercetin and myricetin. The binding constant of herring sperm DNA and four flavones were obtian by Lineweaver-Burk method,respectively. The melting curve were obtainded in this research,the results indicate that the melting behavior of flavone-DNA mixture were different from DNA solution.

Key words: flavones, ultraviolet absorption spectrum, melting curve, herring sperm DNA

HUANG Guo-Xia, LI Jun-Sheng, YAN Liu-Juan. Study on Interaction between Four Flavones and DNA[J]. FOOD SCIENCE, 2008, 29(12): 181-184.

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Study on Interaction between Four Flavones and DNA

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