Intervention Effect of Soy Isoflavones on Hepatic Oxidative Stress and DNA Damage Induced by Carbon Tetrachloride in Mice

doi:10.7506/spkx1002-6630-201401042

Abstract

Abstract:

The intervention effect of soy isoflavones (ISOF) on oxidative stress and DNA damage in a mouse model of
acute liver injury induced by carbon tetrachloride (CCl4) was investigated. Fifty mice were randomly divided into 5 groups
including normal control, model control, bifendate (BFD), high-dose ISOF and low-dose ISOF groups. Animals were treated
once daily for 7 days. At the end of the experiments, CCl4 was injected intraperitoneally to the mice from four groups except
the normal control group. Then, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase
(ALP), albumin (ALB), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), Na+-K+-ATPase,
Ca2+-Mg2+-ATPase, reduced glutathione (GSH) and malondialdehyde (MDA) were determined spectrometrically, and
protein expression of heme oxygenase-1 (HO-1) was assayed through western blotting, and DNA damage was evaluated
by electrophoresis. The results showed that the administration of ISOF reduced serum activities of ALT, AST and ALP GSH-Px;
decrease MDA content in liver; increased total SOD, Mn-SOD, GSH-Px and CAT activities and GSH level in liver; enhanced
Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in liver mitochondria; up-regulated the expression of HO-1 protein; and reduced
DNA damage of hepatocytes in mice with acute liver injury. Therefore, ISOF exerts a protective function on acute liver injury
induced by CCl4 in mice, probably via reducing oxidative stress and DNA damage in liver tissue.

Key words: soy, isoflavones, carbon tetrachloride (CCl4), liver injury, oxidative stress, DNA damage

CLC Number:

R285.5

YIN Xue-zhe1, ZHAO Wen-xi2, JIN Ai-hua1, QUAN Ji-shu2,*. Intervention Effect of Soy Isoflavones on Hepatic Oxidative Stress and DNA Damage Induced by Carbon Tetrachloride in Mice[J]. FOOD SCIENCE, 2014, 35(1): 214-218.

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