Cloning and Sequence Analysis of AURJ3M Gene in Streptomyces aureofuscus SYAU0709

Abstract

Abstract: A pair of primers were designed based on PimM gene sequences published in GenBank and a DNA fragment of approximate 579bp was amplified from Streptomyces aureofuscus SYAU0709 by PCR method. The amplified PimM gene was ligated into vector PMD19 and the recombinant Plasmid was identified by PCR amplification and sequencing. Sequence analysis indicated that the sequence of the cloned AURJ3M gene from Streptomyces aureofuscus SYAU0709 is the 95% similarity as the published PimM gene from Streptomyces natalensis.The fermentation results showed AURJ3M gene could promote Aureofuscin biosynthesis.

Key words: Streptomyces aureofuscus, PimM gene, cloning, sequence analysis

WEI Jie, MENG Xian-Jun, LI Hui, HOU Xiao. Cloning and Sequence Analysis of AURJ3M Gene in Streptomyces aureofuscus SYAU0709[J]. FOOD SCIENCE, 2008, 29(12): 490-493.

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Cloning and Sequence Analysis of AURJ3M Gene in Streptomyces aureofuscus SYAU0709

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