FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (2): 177-181.
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CHU Bi-Hai, DING Xiao-Yu, LI Xue-Xia, HE Jia, DING Ge, LIU Dong-Yang
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Abstract: Aim: To develop a convenient and reproducible molecular marker method to identify the hygienical food of Alisma orientali from its adulterants. Method: PCR products of ITS regions were sequenced and analysed with Clustal X, Mega3.0 and restriction pattern analysis was performed with DNAMAN 4.0. Result: According to the length of rDNA ITS regions, Sagittaria sagittifolia Linn and Ipomoea batatas (L.) Lam were seperated first;further authentication of Alisma orientali from Alisma plantago-aquatica Linn, Alisma canaliculatum A.Braun et Bouche, Alisma nanum D.F.Cui and Colocasia esculenta (L.) Schott can be identifded sucessfully by PCR-RFLP (polymerase chain reaction-restriction fragment length polyrnorphisrn). Conclusion: The length of rDNA ITS (intergenic spacer) regions cannot adequately distinguish hygienical food of Alisma orientali from its adulterants. PCR-RFLP is a simple and rapid method to authenticate the hygienical food of Alisma orientali from its adulterants.
Key words: Alisma orientale (Sam.) Juzep, hygienical food, adulterant, PCR-RFLP, molecular authentication
CHU Bi-Hai, DING Xiao-Yu, LI Xue-Xia, HE Jia, DING Ge, LIU Dong-Yang. Molecular Authentication of Alisma orientali (Sam.) Juzep Food from Its Adulterants by PCR-RFLP[J]. FOOD SCIENCE, 2007, 28(2): 177-181.
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