Abstract: The purpose of this study was to prepare inhibitory peptides of angiotensin converting enzyme (ACE) from Silver carp Hypophthalmichthys molitrix with flavorzyme. The optimal hydrolysis parameters for flavorzyme were determined by orthogonal design, and then the hydrolysates were fractionated by Sephadex G-15. The results showed that the optimal hydrolysis conditions that could produce the hydrolysates with the highest inhibitory activity (I) on ACE for flavorzyme are temperature 50℃, pH8.0, fish/water ratio 1:6, enzyme dosage 5000U/g pro, and time duration 12h, with the degree of hydrolysis (DH) as 34.40%, the rate of nitrogen recovery as 92.01%, and the inhibitory activity (I) as 66.52%. In vitro experiments results showed that the peptides with high or low molecular weights have low capability to inhibit ACE activity, and only the short chainpeptides with a certain molecular weights range show high activity. The fraction 3 has the maximal inhibitory activity of 63.04%.

Key words:  , Silver carp (Hypophthalmichthys molitrix)； hydrolysis； flavorzyme； ACE inhibitory peptides； antihy- pertensive peptides；