Effect of Magnetic Fields on Superoxide Dismutase Activity Using Response Surface

Abstract

Abstract: The effect of three variables (temperature, magnetic intensity, and exposure time) on the activity of superoxide dismutase (EC 1.15.1.1) isolated from Saccharomyces cerevisiae was studied by Response Surface Methodology (RSM). This multivariate methodology offers an empirical approach to the study of enzyme assays and allows to detect the interaction between different variables of the system. Analysis of variance (ANOVA) showed that a second order polynomial model (SOP) generated response surface and contour plots, and the model may predict the experimental data. Lack-of-fit tests did not result in a significant F-value. Determination coefficients (R2) were greater than 94%. The optimum treating parameters were obtained as: temperature, 15.5℃; magnetic intensity, 0.95T; time of magnetic field exposure, 64.5 minutes.

Key words: superoxide dismutase, Saccharomyces cerevisiae, response surface methodology

WANG Shan-shan,LI Bing,LI Lin. Effect of Magnetic Fields on Superoxide Dismutase Activity Using Response Surface[J]. FOOD SCIENCE, 2006, 27(12): 62-66.

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Effect of Magnetic Fields on Superoxide Dismutase Activity Using Response Surface

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