Cloning and Sequence Analysis to Fimbrial Subunit Gene(fimA) of Edwardsiella tarda

Abstract

Abstract: Fimbrial subunit gene (fimA) of Et, XA and EA isolates were cloned and sequenced. The sequence analysis showed that the fimbrial subunit gene of Et, XA and EA isolates contained 534 nucleotides, encoding a protein of 117 amino acids. The similarity of nucleotide (nt) sequence of fimA gene among Et, XA and EA isolates were 99%. The nucleotide and amino acid(aa) sequence of fimA gene of these isolates shared 76% to 77% and 81% to 82% similarities to that of other Edwardsiella tarda isolates, respectively.

Key words: Edwardsiella tarda, FimA, cloning, sequence analysis

GUO Li-Xin, Li-Shou-Song, Jiang-Shu-Xun, Chen-Wen-Bing, Shao-Bi-Ying, Yang-Jie. Cloning and Sequence Analysis to Fimbrial Subunit Gene(fimA) of Edwardsiella tarda[J]. FOOD SCIENCE, 2006, 27(11): 45-48.

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Cloning and Sequence Analysis to Fimbrial Subunit Gene(fimA) of Edwardsiella tarda

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