Researches on Properties of Polyphenoloxidase(PPO)Ex-tracted from Lettuce(Lactuca Sative)

Abstract

Abstract: The paper mainly researched the prop erties of polyphenoloxidase extracted fromlettuce.It studied the PPOactivities with catechol as substrate in different pH,temperature,enzyme concertrations,substrate c oncer-trations and inhibitors.The results showed that there existed two kinds of isoenzymes in lettuce PPO with its opti-mumpHandtemperature as 5,7and50℃,respectively and the Kmand max as 7.125×10 -3 mol /L,1.4625×10 -2 U /min,respectively.The orders of i nhibition effects fromstrong to wea k were fromNaHSO 3 ?PA?VC to EDTA.

Key words: lettuce, polyphenoloxidase, brow ning, dynamics properties

CHEN Xue-Hong, QIN Wei-Dong, GAO Hui, ZHAO Jia-Ji. Researches on Properties of Polyphenoloxidase(PPO)Ex-tracted from Lettuce(Lactuca Sative)[J]. FOOD SCIENCE, 2003, 24(3): 29-32.

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Researches on Properties of Polyphenoloxidase(PPO)Ex-tracted from Lettuce(Lactuca Sative)

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