Study on Esterase Used in Biosensor for Pesticide Residue Assay

Abstract

Abstract: Bothanimal -esterase and plant -esterase can be inhibited by pesticide.So,plant -esterase,as well as animal -esterase,can be used to detect pesticide residue.In this paper,plant -esterases,compared with animal -esterase were studied on activity,inhibition degree and relate d properties.The results of experim ent showed that the activity of plant -esterases and inhibition degree on pla nt -esterases was almost the same as an-imal -esterases.Both as plant -este rase,corn esterase’ s activity was much lower than wheat’ s.So it was not suitable to detect pesticide by usin g corn esterase.The pH effect was als o studied.The optimal pH value for plant -esterase was 6.5,and that for animal -esterase was 7.5.

Key words: pesticide residue, colorimetry, pl ant -esterase, animal -esterase

WANG Zhong-Hai, XU Fei. Study on Esterase Used in Biosensor for Pesticide Residue Assay[J]. FOOD SCIENCE, 2003, 24(1): 21-23.

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Study on Esterase Used in Biosensor for Pesticide Residue Assay

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