PCR Detection for Some Components Extracted from Plant in Food

Abstract

Abstract: Total DNA was extracted from soybean-milk powder and potato,maize,tamato and their procced food. The plant derived components in food were identified by PCR detection for plant endogenous genes such as soybean lectin gene,potato patatin gene,maize IVR gene and tomato PG gene as well as their command genes such as rbcL gene of plant chloroplast and 18S rDNA fragment. Duplex PCR of above very endogenous gene together with 18S rDNA or rbcL gene respectively were also carried out. The results indicated single PCR and duplex PCR could be applied to identify plant derived components in food steadily.

Key words: food, plant derived component, PCR

CHEN Wen-Bing, Shao-Bi-Ying, Jiang-Shu-Xun, Yang-Jie, Li-Shou-Song, Guo-Li-Xin. PCR Detection for Some Components Extracted from Plant in Food[J]. FOOD SCIENCE, 2006, 27(11): 404-408.

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PCR Detection for Some Components Extracted from Plant in Food

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