Abstract: β - glucosidase (EC. 3.2. 1.21) production conditions of Aspergillus niger (As. n. XD - 1) were investigated through single factor experimentation and orthogonal test. The results showed that solid state fermentation was the suitable way of β - glucosidase production were 2 portions of bagasse, 3 portions of wheat bran, 0. 8% yeast extract, 3 times of water, initial natural pH value, and fermentation at 28() for 4d. Under these conditions, the activity of β - glucosidase could reached 23. 86U/ml.

Key words: Aspergillus niger , β- glucosidase , Produced condition