Abstract: Seed and young stem as well as young leaf of Ginkgo biloba were used to induce callus. Optimum conditions and hormone combinations were sifted for Ginkgo callus growth and subculture. The biosynthesis amount of flavonoids was assayed in the callus suspension culture. The results showed: (1) The culture medium for induced and subculture of Gingko biloba callus was the MS culture medium by adding 3. 0mg/L NAA and 1. 0 mg/LBA. (2)0. 1% phytic acid has inhibited the callus browning and promoted the callus growth. (3) Adding 5. 0% of sucrose in culture medium increased the callus growth and the growth time of the callus reached amaximum value during subculture 40 to 45 days. (4) Gingko callus growth rate was 6. 8 times higher during suspension culture and biosynthesis amount of the flavonoids reached 76 mg/L.

Key words: Ginkgo , Callus , Induced , Suspension cultures , Flavonoids