Study on Extracting Yeast Saturated Fatty Acids with Urea Inclusion Method

Abstract

Abstract: The fat-producing optimum conditions of high-yield yeast（Candida Yeast-1-1）were studied in this experiment.An optimum method of yeast fat urea inclusion complex of saturated fatty acids using multi-criteria cross experiment was obtained. The optimized liquid fermentation mediums are as follows:0.02% MnCl2,0.03% FeSO4,0.3%（NH4）2SO4,3%C6H6O and pH6.5,at 120 r/min and 30℃.The optimum extracting conditions of yeast fat urea inclusion complex are:ratio of urea and fatty acids（3:1）,refluxing time（30 min）and cooling temperature（0℃）.

Key words: unsaturated fatty acids, urea inclusion method, orthogonal test

ZHAO Feng-Li, CHEN Rui, LU Cui-Wen. Study on Extracting Yeast Saturated Fatty Acids with Urea Inclusion Method[J]. FOOD SCIENCE, 2007, 28(5): 142-145.

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Study on Extracting Yeast Saturated Fatty Acids with Urea Inclusion Method

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