Abstract: A method for determination of cholesterol in extracts from antler velvet by supercritical carbon dioxide (SC-CO2) extraction was presented. Cholesterol was assayed by gas chromatography with flame ionization detection, where oven temperature was controlled with a temperature elevation program set at 278 ℃ for 5 min, elevated to 286 ℃ at the rate of 2.5 ℃/min for 6 min, and then elevated to 305 ℃ at the rate of 40 ℃/min maintained for 3 min. The temperature at injector port and detector was set at 295 ℃ and 305 ℃ respectively. The flow rates were 30 ml/min for nitrogen, 47 ml/min for hydrogen, and 400 ml/min for air. The calibration curve is linear with the ranges of 0.20～1.00μg for cholesterol and the regression equation is Y=106X- 54842(r=0.9989). The recovery is 100.4% with RSD 3.3%(n=5).

Key words: gas chromatography(GC), cholesterol, antler velvet, supercritical carbon dioxide extraction