FOOD SCIENCE ›› 2002, Vol. 23 ›› Issue (7): 47-52.

Previous Articles     Next Articles

Study on Extraction Purification and Esteripication of Eritadenine

 SUN  Pei-Long, WU  Xue-Qian, JI  Pei-Jun, ZHAO  Pei-Cheng, ZHAO  Ming-Zhu   

  • Online:2002-07-15 Published:2011-12-31

Abstract: The studyabouttheextraction,purificationandesterificationoferitadeninewas reportedinthis paper.T he optimumextracting technique was veritied as follows:100grams dry stem of Lentinus edodes were soaked and pu lped with8~10times of75%(v /v)alcoholfor 6hours,thenextractedf or 2hat83~85℃.After being concentr ated,75ml eritadenine solutionwas obtained,where the concentrationwas 200~220mg /100ml.Then,purifying the conce ntrate with two ion exchange chromatographic column,3ml eritadenine refined s olution was obtained,withthe concentration was of 1.9%.Desiccating the solutio n,the eritadenine powder could be ga ined.The refined solution added wit h 300times of95%(v /v)alcoholcatalyzedbyconcentratedv itriolwas esterifiedfor4hours at70℃.Therateofesterification was 70%.

Key words: Lentinus edodes , Eritadenine , Extraction , Purification , Esterification