Study on the Cloning of Calpain Activator cDNA and the Analysis of Its Sequence

Abstract

Abstract: Calpain is the main proteinase system that would regulate the proteolysis postmortem. To promote the activity of calpain so as to improve the tenderness of meat is always the important topic of scientists who have studied the meat treatment.The recent study indicated that the calpain activator was the new component of calpains, and the chief regulator of calpain. Calpain activator could activate calpain , and improve the activity of calpain to about a multiple of 400 .Cloning of calpain activator cDNA by using the method of molecular biology is another new topic in food biotechnology field, and it establishes the base of biotechnique of meat tenderization.It could also improve the productivity. This study has cloned the calpain activator cDNA by using the PCR method, and analyzed the sequence of this protein. Comparison of this sequence with that of UK114 showed that the Variance between them was only 5bp.

Key words: Calpain activator , Cloning , Tenderization

CHANG Hong, NAN Qing-Xian . Study on the Cloning of Calpain Activator cDNA and the Analysis of Its Sequence[J]. FOOD SCIENCE, 2002, 23(5): 25-29.

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Study on the Cloning of Calpain Activator cDNA and the Analysis of Its Sequence

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