Study on Optinal Conditions of Immobilized Chifosanase on Chitin

Abstract

Abstract: Chitosanase was immobilized on chitin with glutaraldehyde by cross-link reaction,the immobilization conditions and characterization of the immobilized enzyme were studied.The optimal conditions for immobilization were as follows:0.1g chitin was treated with 5ml 5% solution of glutaraldehyde,then 2mg chitosanase was immobilized on the carrier.Optimal temperature and pH for the immobilized enzyme were 60℃ and 4.0 respectively.Kinetic parameter Km was 17.66g/L.The immobilized enzyme showed good temperature stability,and its operation and storage stability were also very good.

Key words: , Chitin Immobilization Chitosanase Chitosan；

Zeng-Jia, Zheng-Lian-Ying. Study on Optinal Conditions of Immobilized Chifosanase on Chitin[J]. FOOD SCIENCE, 2001, 22(10): 21-24.

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Study on Optinal Conditions of Immobilized Chifosanase on Chitin

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