Method Study on Assaying EPA/DHA in Fish Oil and its Products

Abstract

Abstract: A method for determination of eicosaqpentaenoic acid and docosahexaenoic acid in fish oil and its products by gas chromatography/mass spectrometry was developed.The sample was first extracted by n-hexane,then the fatty acid was methylated with potassium hydroxide-methanol(KOH-MeOH).The obtained fatty acid methyl esters were determined and confirmed by gas chromatograph equipped with MSD on DB-17 fused silica capillary column(30m×0.25mm×0.10μm),using normal method.The recovery was from 93.3% to 102.1% CV(n=10) was from 2.83% to 3.42%.The method was simple,rapid,accruate and applicable to determine practical samples with satisfactory results.

Key words: , Eeicosapentaenoic acid(EPA) Docosahexaenoic acid(DHA) Fish oil and its products Gas chromatography/mass spectrometry；

MOU Jun, Qu-Zhong-Wen. Method Study on Assaying EPA/DHA in Fish Oil and its Products[J]. FOOD SCIENCE, 2001, 22(10): 76-78.

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Method Study on Assaying EPA/DHA in Fish Oil and its Products

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