Comparison of Two Methods for the Determination of Condensed Tannins in Wine

Abstract

Abstract: Objective: Two most common methods for the determination of condensed tannins in wine: methylcellulose precipitation (MCP) assay and Adams-Harbertson (A-H) protein precipitation assay were compared with each other to find a rapid and accurate method. Methods: Condensed tannins in 32 red and 12 white wine samples from different varieties and different growing regions were determined separately by MCP assay and A-H assay to obtain average condensed tannin content and coefficient of variation. Determination of condensed tannin content in these samples was also carried out using HPLC-MS. In addition, total phenolic content (expressed as absorbance at 280 nm) was spectrometrically determined. Results: Both assays were suitable for the determination of condensed tannins in red wine rather than white wine. The MCP assay, although accurate, did not show a significant variation. The average condensed tannin content measured by the MCP assay was 9.13 times higher than that measured by the A-H assay, and a good linear correlation was observe between both assays (R2 = 0.6029, P＜0.01). Moreover, the MCP assay showed a good linear correlation with HPLC-MS (R2 = 0.7733, P＜0.01) but there was no significant correlation between the A-H assay and HPLC-MS (R2 = 0.4843, P＜0.01). A significant correlation between the content of condensed tannins determined by the MCP assay and the content of total phenols was observed (R2 = 0.9095, P＜0.01), but the content of condensed tannins determined by the A-H assay and the content of total phenols revealed a poor correlation with each other (R2 = 0.2872, P＜0.01). Conclusion: Both assays can be used to determine condensed tannins in red wine. However, the MCP assay is a better method for the determination of condensed tannins in case that the content of total phenols is determined based on absorbance at 280 nm.

Key words: wine, condensed tannins, MCP assay, A-H assay, HPLC-MS, total phenols

CLC Number:

中图分类号：TS261.7

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