Optimization of Ion Precipitation Conditions for Separation of Quercetin from Fructus Forsythiae

Abstract

Abstract: In order to develop a method for the separation of quercetin from ethanol extract from Fructus Forsythiae, the effects of precipitant dose, temperature and stirring time on quercetin yield were explored. The final product was analyzed by HPLC using quercetin standard. The results showed that the adding 0.60 g of sodium carbonate and 1.00 g of zinc acetate and stirring for 40 min provided a quercetin yield of 4.20% from the 60% ethanol extract from 4.00 g of Fructus Forsythiae. The resulting precipitate showed an HPLC profile similar to that of quercetin standard.

Key words: Fructus Forsythiae, quercetin, zinc ion precipitation method, separation

CLC Number:

TS209

References

[1] 郭强,王智民,林丽美,等.连翘属药用植物化学成分研究进展[J].中国实验方剂学杂志, 2009,15(5):74-79.
[2] 栾兰,王钢力,林瑞超.连翘水提物化学成分研究[J].中草药,2010,41(6):883-885.
[3] 中国科学院昆明植物研究所.云南植物志[M].北京:科学出版社,1986:61.
[4] YUKO Hirose, TOMOYUKI Fujita, SEIICHI Matsugo. Oxidative products from quercetin during lipid peroxidation[J]. ITE Battery Newsletter, 2001,2(6): 825-828.
[5] Walle, Thomas. Absorption and metabolism of flavonoids[J]. Free Radical Biology and Medicine,2004,36 (7):830-831.
[6] ELIZABETA Dimitrieska-Stojkovic, ZORAN Zdravkovski. Supercritical Fluid Extraction of Quercetin and Rutin from Hyperici Herba[J]. Journal of liquid chromatography and related technologies, 2003,26(15):2517-2533.
[7] JING Limin, YOU Jingyan, YAO Shuang, et al. Microwave-assisted Extraction of Rutin and Quercetin from Flos Sophorae[J]. Chemical Research in Chinese Universities, 2004,20(6):703-706.
[8] MERKEN Howardmark, BEECHER Garyr. Liquid chromatographic method for the separation and quantification of prominent flavonoid aglycones[J]. J Chromatography A, 2000,897(1):177-184.
[9] 龚受基,黄耀锋,滕翠琴,等.大田基黄中槲皮素含量分析和总黄酮的提取工艺优化[J].食品科技,2010,35(5):199-205.
[10] 张颖,巩学勇,冀海伟,等.翻白草中槲皮素的提取工艺优化及含量测定[J].中国药房,2008,19(30):2345-2347.
[11] 蒋立娣,宣贵达,吴好.桑叶提取物中槲皮素和山萘酚的含量测定[J].浙江大学学报,2009,36(6):705-708.
[12] 蒋建平,陈洪,汪秋安,等.茶多酚的离子沉淀法提取及其成分分析[J].株洲工学院学报,2004,18(5):53-57.
[13] 许光明,彭新君,王彩君,等.三叶虫茶中多酚类物质离子沉淀法提取工艺研究[J].湖南中医药大学学报,2007,27(2):46-48.
[14] 陶洁.金属离子沉淀法处理含酚废水及回收酚类物质的研究[J].科技咨询导报,2007,(23):116-117.
[15] 吕程丽,欧阳玉祝,梅杰,等.CO2超临界萃取葛根总黄酮的研究[J].食品与发酵科技,2009,45(5):21-23.
[16] 阎素清,柴保臣,贾小燕,等.懈皮素-钼配合物的化学合成[J].郑州大学学报,2008,43(4):782-785.