Optimization of Extraction Conditions for Superoxide Dismutase from Sheep Blood by Thermal Denaturation Coupled with CuSO4

Abstract

Abstract: The purpose of this study was to optimize the extraction of superoxide dismutase (SOD) from sheep blood by thermal denaturation coupled with CuSO4. Main process conditions that influence SOD specific activity were optimized using one-factor-at-a-time and orthogonal array design. The results showed that thermal denaturation at 65 ℃ for 15 min in the presence of 2.5% CuSO4 in 10 mmol/L aqueous solution and subsequent addition of a 1.25-fold excess of acetone resulted in an SOD specific activity of 2338.9 U/mg. This method was simple, low cost and suitable for industrialized production.

Key words: superoxide dismutase (SOD), enzyme activity, CuSO4, thermal denaturation

CLC Number:

Q814

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