Isolation and Purification of Casein Glycomacropeptide with Chitosan

Abstract

Abstract: This study aimed to investigate process conditions for the isolation of casein glycomacropeptide (CGMP) from whey powder by chitosan adsorption. The effects of final pH, final chitosan concentration and adsorption time as well as NaCl concentration and pH for CGMP elution on the efficacy of chitosan for isolating CGMP were explored. The optimum conditions for the isolation of CGMP were determined as follows: chitosan was added to a final concentration of 2 g/L and the pH was adjusted to 4.6 for adsorption of CGMP by chitosan for 60 min; the adsorbed CGMP was then eluted using 2 mol/L NaCl solution alkalified to pH 9; finally the resultant eluate was pooled, desalinate by means of ultrafiltration, concentrated and lyophilized. As a result, CGMP powder was obtained. The results of scale-up experiments showed that the recovery rates of CGMP and sialic acid were 83.3% and 70.64%, respectively and that the CGMP obtained contained 10 mg of sialic acid per gram of sample. This CGMP was composed of a multimer with respective molecular weights of 22 ku and 30 ku as demonstrated by gel electrophoresis analysis.

Key words: chitosan, casein glycomacropeptide, isolation and purification, whey powder, sialic acid

CLC Number:

中图分类号： TS252.1

References

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