Biocatalytic Production of Glycerophosphatide Choline in Non-aqueous Phase

Abstract

Abstract: This study was undertaken to optimize reaction conditions for the biocatalytic deacylation of phosphatidylcholine (PC) by lipase to glycerol-3-phosphocholine (GPC) in non-aqueous phase. The optimal reaction conditions were found to be reaction at 40 ℃ and pH 5.0 for 6 h with an enzyme dose of 12.5 mg/mL in the presence of 4% water. Moreover, in order to increase the utilization rate of lipase and reduce solvent consumption, additional PC at a concentration of 0.437 g/mL was added in a proportion of 50% relative to the initial amount after 1.5 h during the reaction process. Under these process conditions, the average yield of GPC after aqueous extraction of reaction products was 97% (n = 3) as determined by HPLC. Thus, a feasible method to prepare GPC has been established.

Key words: lipase, phosphatidylcholine, glycerophosphatide choline, non-aqueous phase

CLC Number:

TS218

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