Cell Wall Disruption of Rape Bee Pollen by Cellulase

Abstract

Abstract: The effects of solid-to-liquid ratio, enzyme loading, temperature and hydrolysis time on the efficacy of cellulase for cell wall disruption of rape bee pollen were examined in this study. One-factor-at-a-time and orthogonal array design methods were used to optimize the experimental conditions based on cell wall disruption rate and polysaccharide yield. The optimal conditions for cell wall disruption of rape bee pollen were found to be hydrolysis at 47.5 ℃ for 5.0 h with a solid-to-liquid ratio of 1:50 (g/mL) and an enzyme loading of 1.75%, resulting in a cell wall disruption rate of 85.72% and a polysaccharide yield of 18.85%.

Key words: rape bee pollen, cellulase, cell wall disruption, microscopic observation, polysaccharide

CLC Number:

TQ929.2

References

[1] 任向楠,张红城,董捷.蜂花粉破壁的研究进展[J].食品科学,2009,21:380-383.
[2] 王芙蓉.油菜花粉的破壁方法及其多糖、黄酮提取纯化工艺研究[D].武汉:华中农业大学.2007.
[3] 刘建涛,赵利,苏伟.蜂花粉生物活性物质的研究进展[J].食品科学,2006,27(12):909-911.
[4] 张红城,程蒙,董捷,孙庆申.六种蜂花粉中酶活性的研究[J].食品科学,2009,30(21):229.
[5] 周小玲,王永宁.青海油菜蜂花粉的破壁及黄酮的测定[J].青海师范大学学报(自然科学版),2009(4):79.
[6] 王永宁,卢挺,石玉平.油菜蜂花粉酶法破壁研究[J].天然产物研究与开发,2004,16(3).
[7] 郝功元,吴彩娥,李婷婷,杨剑婷.银杏花粉不同破壁方法的比较分析[J].南京林业大学学报(自然科学版),2009,33(4):81-85.
[8] 阮征,邓泽元,吴龙耀等.HPLC法测定油菜蜂花粉中黄酮含量及六种破壁方法对黄酮提取的影响[J].食品科学,2008,29(10):455-458.
[9] 唐维,张星海,花粉破壁方法的研究进展[J].食品与发酵工业,2003,29(2):86-92.
[10] 何余堂,王婷,于洋等.玉米花粉混合破壁法的研究[J].食品科学,2006,27(10):282-284.
[11] KROON G H, van PRAAGH J P, VELTHUIS H H W. Osmotic shock as a prerequisite to pollen digestion in the alimentary tract of the worker honey bee[J].Journal of Apicultural Research,1974,13:177-181.
[12] 胡筱波,徐明刚,吴谋成.温差破壁法对油菜蜂花粉中主要营养素含量的影响[J].食品科学,2005,26(10):120-124.
[13] 杨晓萍,余志勤.油菜花粉破壁方法研究[J].华中农业大学学报,2004,23(6):671-672.
[14] 朱文学,龚明贵.杜仲雄花粉酶法破壁的研究[C].2007年全国包装与食品工程学术年会.宜昌2007,10,18:109-115.
[15] 李飞.山楂蜂花粉多糖提取、分离纯化、结构鉴定和生物活化的研究[D].北京:北京化工大学.2009.