Whole Genome Shuffling of Bacillus subtilis for Enhanced Protease Production

Abstract

Abstract: Whole genome shuffling technique was used to enhance protease production by Bacillus subtilis B4. A candidate mutant library was constructed by UV irradiation of Bacillus subtilis B4. Based on the optimum conditions for protoplast preparation and regeneration, multi-parental whole genome shuffling was carried out with 4 mutant strains (BRS1, BRS2, BRS5 and BRS6) by PEG mediated protoplasts fusion. Then BRS1, BRS5 and BRS6 were identified by biparental inactivation method as 3 excellent strains with greatly improved protease activity and good genetic stability. The maximum activity was as high as 2175.81 U/mL, which was increased 3.01 fold compared with the initial strain B4.

Key words: Bacillus subtilis, protease, genome shuffling

CLC Number:

Q819

References

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