Effects of Different Kinds of Amino Acids on Mycelial Growth and Cordycepin Content in Cordyceps militaris

Abstract

Abstract: In this study, we investigated the effect of individual addition of 20 kinds of amino acids at a concentration of 0.2 g/100 mL to liquid PDA medium for the cultivation of Cordyceps militaris in shake flasks on mycelial growth and cordycepin production. 16 of the 20 kinds of amino acids had obvious mycelial growth-promoting effects on Cordyceps militaris. The top five most potent growth promoters included arginine, lysine and histidine as basic amino acids as well as aspartic acid and glutamic acid as acidic amino acids, which resulted in 2.10, 1.82, 1.63, 1.48 and 1.40-fold increases in mycelial biomass, respectively. Moreover, each of the 20 kinds of amino acid could significantly enhance the accumulation of cordycepin in Cordyceps militaris mycelia. Addition of the basic amino acids arginine and histidine resulted in 2.91 and 2.90-fold increases in cordycepin content in Cordyceps militaris mycelia, respectively. Upon addition of the polar polar amino acids threonine and asparagine, 2.43 and 2.42-fold increases, respectively, were observed, while cordycepin content in Cordyceps militaris mycelia presented a 2.05-fold increase when the non-polar amino acid alanine was added.

Key words: Cordyceps militaris, cordycepin, amino acid, liquid-state cultivation, mycelia

CLC Number:

References

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