Purification and HPLC-ELSD Determination of Gypenosides from Gynostemma pentaphyllum (Thunb.) Makino Leaves

Abstract

Abstract: D101 macroporous resin was used to purify gypenosides from crude extract of Gynostemma pentaphyllum (Thunb.) Makino leaves. The effects of sample concentration and ethanol concentration for desportion on the adsorption and desorption of total saponins were investigated to find optimal chromatographic conditions for enriching and separating ginsenosides Rb1, Rb3 and Re. HPLC-ELSD was used to analyze eluates obtained with different concentrations of ethanol. The optimal purification conditions were determined as follows: 6 mg/mL sample concentration and desorption with 50% ethanol aqueous solution, resulting in a total saponin purity of 92.76%.

Key words: gypenosides, macroporous resin, high performance liquid chromato graphy (HPLC), evaporative light scattering detector (ELSD)

CLC Number:

TS207.3

[1]	HUANG Dongwei, GU Guizhang, HU Kena, GAO Xingjie, ZHANG Jinjie, YANG Wenge, XU Dalun. Detection of Guanine in Fish Flesh by Protein Adsorption onto Macroporous Resin Combined with Surface-Enhanced Raman Spectroscopy [J]. FOOD SCIENCE, 2021, 42(18): 298-305.
[2]	CHEN Lin, Lü Yangjun, ZHANG Haihua, ZHU Yuejin, WANG Yuefei, ZHANG Shikang. Use of Macroporous Resin LP-8 for Preparing Tea Polyphenols Rich in Esterified Catechins [J]. FOOD SCIENCE, 2019, 40(7): 68-73.
[3]	SHUI Longlong, CAO Lili, PANG Min, PAN Lijun, BAO Sai, LI Jinhong, JIANG Shaotong. Optimization of Process Parameters for Immobilization of Phospholipase A1 onto Macroporous Resin and Its Application for Degumming Rapeseed Oil [J]. FOOD SCIENCE, 2018, 39(4): 241-247.
[4]	ZHAO Ziying, PIAO Chunhong, WANG Yuhua, LIU Junmei, YU Hansong, DAI Weichang, TANG Yufang, WANG Jing, LIU Dailin. Isolation and Anti-Diabetic Activity in Vitro of Flavonoids from Buckwheat Hull [J]. FOOD SCIENCE, 2018, 39(3): 21-27.
[5]	LI Xia, ZANG Xueli, XU Yibo, WANG Dawei. Optimization of Purification of Flavonoids from Mung Bean Coat by AB-8 Macroporous Resin and Comparison of Antioxidant Capacity of crude and purified Flavonoids [J]. FOOD SCIENCE, 2018, 39(10): 283-290.
[6]	YU Zeyuan, ZHAO Jianhui, LI Xingguo, XU Yaqin, TANG Xue, YANG Yu. Isolation and Purification of Anthocyanin from Blueberry by Sequential Medium Pressure Column Chromatography on Macroporous Resin and Sephadex LH-20 [J]. FOOD SCIENCE, 2018, 39(1): 118-123.
[7]	ZHANG Xuan, HAN Tao, YAN Tingcai, BAI Bing, WANG Yanchao, MA Rongshan. Screening of Adsorbents for the Removal of Plasticizer from Luzhou-Flavor Base Liquor [J]. FOOD SCIENCE, 2017, 38(5): 92-97.
[8]	ZHU Jie, WANG Hongbao, KONG Jiajun, SONG Xiaofei, TAO Shutian. Purification and Antioxidant Activity of Polyphenols from Young Pear Fruits [J]. FOOD SCIENCE, 2017, 38(5): 14-20.
[9]	MIAO Xiugang, YU Xiang, ZHANG Beibei, ZHANG Jingfang, LIANG Junbin. Purification of Flavonoids from Toona sinensis Leaves with NKA-9 Macroporous Resin [J]. FOOD SCIENCE, 2016, 37(8): 32-38.
[10]	LI Bin, GAO Ningxuan, LIU Hui, MENG Xianjun, ZHANG Qi, JIAO Xinyao, LI Enhui1, CHOU Shurui, ZHANG Ye, CHEN Shifu. Macroporous Adsorption Resin for the Purification of Polyphenols from Aronia melanocarpa Berries [J]. FOOD SCIENCE, 2016, 37(16): 69-74.
[11]	ZHANG Di, ZENG Qingmei, WANG Lin, YAN Chunming, GAO Ya. Optimization of Microwave-Assisted Enzymatic Extraction of Gypenosides from Gynostemma pentaphyllum [J]. FOOD SCIENCE, 2016, 37(12): 1-6.
[12]	LIU Yutao, YANG Xiaoming*. Enrichment and Purification of Ginkgo biloba Leaf Polyprenols by NKA-Ⅱ Macroporous Resin [J]. FOOD SCIENCE, 2015, 36(4): 63-67.
[13]	CHEN Xiaoxia1, SONG Yuan2, ZHANG Jibai3, WANG Zengli1,*. Purification of Flavonoids from Cordyceps militaris by Macroporous Adsorption Resin [J]. FOOD SCIENCE, 2015, 36(4): 68-73.
[14]	YANG Yunshu, LI Rong, JIANG Zitao. Optimization of Microwave-Assisted Extraction by Response Surface Analysis and Purification of Flavonoids from Choerospondias axillaris Fruit [J]. FOOD SCIENCE, 2015, 36(22): 18-24.
[15]	DONG Qi1, GAO Shan1, CAO Longkui1,2,*. Purification of Polyphenols from Inonotus obliquus by Macroporous Resin and Its Component Analysis [J]. FOOD SCIENCE, 2015, 36(22): 131-136.

Purification and HPLC-ELSD Determination of Gypenosides from Gynostemma pentaphyllum (Thunb.) Makino Leaves

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments