Abstract: Objective: To develop an analytical method for the simultaneous determination of seven organic acids in fruit vinegar by high-performance liquid chromatography (HPLC). Methods: Organic acids in fruit vinegar were extracted with water, cleaned up using an LC-SAX solid phase extraction cartridge, separated using an Atlantis    T3 C18 column and detected using a diode-array detector. Results: Seven organic acids were separated within 15 minutes. The recovery rates of seven organic acids were in the range of 97.5%－105.1% with RSD of less than 1.31%. The limits of detection were 0.30－1.80 μg/mL. Conclusion: This method provides a simple, rapid, accurate and suitable method for the simultaneous determination of organic acids in fruit vinegar.

Key words: fruit vinegar, organic acids, high performance liquid chromatography, solid phase extraction