in vitro Physiological Functions of Bioactive Peptides from Grass Carp Meat

Abstract

Abstract: In this study, bioactive peptides were prepared from grass carp meat by enzymatic hydrolysis and ultrafiltration. Their angiotensin I-converting enzyme (ACE) inhibitory activity was determined by high performance liquid chromatography (HPLC) and antioxidant activity was evaluated by DPPH radical scavenging and ORAC (oxygen radical absorbance capacity) assays. The results showed that the prepared bioactive peptides had strong ACE inhibitory activity with IC50 of 0.229 mg/mL. The concentration needed to scavenge 50% DPPH radicals was 7.630 mg/mL and an ORAC value of 1317.962 Trolox μmol/g was achieved. Thus, grass carp meat is a good source of ACE inhibitory peptides.

Key words: grass carp peptides, ACE inhibitory activity, DPPH free radical, ORAC

CLC Number:

TS254.9

References

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