Abstract:

To investigate the desalination of grass carp peptides (GCP) with macroporous adsorption resinDA201-C. Methods: Ash and peptide contents, molecular weight and amino acid composition of GCP were analyzed beforeand after desalination. Desalination efficiency, angiotensin I-converting enzyme (ACE) inhibitory activity, 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and ferric reducing antioxidant potential (FRAP) were used to evaluatehow well GCP was desalinated. The results showed that the desalination efficiency of GCP was 82.02%. The peptidefragments and hydrophobic amino acids were enriched in desalinated GCP. Meanwhile, the ACE inhibitory activity wassignificantly higher than that of native GCP, showing a decrease in IC50 from 0.36 to 0.23 mg/mL, and the antioxidantactivity was also increased showing an IC50 of 4.48 mg/mL compared to 5.59 mg/mL for native GCP. The desalinationresulted in an increase in the FRAP value of GCP from 1.018 to 1.261. Conclusion: The use of macroporous adsorption resinDA201-C provides a simple and effective method to desalinate GPC.

Key words: macroporous adsorption resin, grass carp peptides, desalination