Purification and Amino Acid Composition of Peptides from Soybean Byproduct Protein Hydrolysate from Aqueous Enzymatic Extraction of Soybean Oil

doi:10.7506/spkx1002-6630-201622016

Abstract

Abstract: In this paper, the adsorption performances and purification efficiencies of 8 macroporous adsorption resins (MARs) for peptides from soybean byproduct protein hydrolysate from aqueous enzymatic extraction of soybean oil were comparatively evaluated by static adsorption and desorption experiments in order to find the best one among these MARs. Further, the effects of feeding volume, feeding rate and desorbent concentration on the purification efficiency of MARs were investigated. Results showed that DA201-C resin had the highest adsorption capability for soybean peptides among 8 resins tested. The optimum dynamic adsorption conditions of DA201-C resin were determined as follows: sample loading volume, 140 mL; feeding flow rate, 1.5 mL/min; 350 mL of water as washing solvent; and fractional elution with 25%, 50%, 75% and 100% using 80 mL of each concentration gradient at 2 mL/min flow rate. Under these conditions, the purity of the purified soybean peptides reached above 80% with a total recovery of 95.65% and a removal rate of more than 90% and 51% for salt and sugar impurities, respectively, and the adsorption capacity of DA201-C resin was 13.32 mg/g. Four soybean peptide fractions were separated by fractional elution with ethanol. A fraction eluted with 75% ethanol, SP-DA75, showed the highest oxygen radical adsorption capacity (ORAC) among the four fractions. Moreover, there was a correlation between antioxidant activities of peptide fragments and their contents of hydrophobic amino acids and acidic amino acids. In conclusion, DA201-C resin exhibited excellent adsorption performance and could be applied as an effective method to purify soybean peptides.

Key words: aqueous enzymatic extraction, soybean peptide, macroporous adsorption resin, purification, amino acid composition

CLC Number:

TS229

ZHANG Qiaozhi, BI Shuang, MA Wenjun, LI Yang, SUI Xiaonan, JIANG Lianzhou*. Purification and Amino Acid Composition of Peptides from Soybean Byproduct Protein Hydrolysate from Aqueous Enzymatic Extraction of Soybean Oil[J]. FOOD SCIENCE, 2016, 37(22): 112-118.

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